Navegando por Assunto "Carotenoids"

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Acesso aberto (Open Access)
Adsorção e dessorção supercrítica de carotenos e antioxidantes do óleo de buriti (Mauritia flexuosa, Mart) em leito de γ- Alumina
(Universidade Federal do Pará, 2012-03) CUNHA, Marcos Augusto Eger da; FRANÇA, Luiz Ferreira de; http://lattes.cnpq.br/6545345391702172; MACHADO, Nélio Teixeira; http://lattes.cnpq.br/5698208558551065
In this work we investigated the enrichment of antioxidants buriti oil (Mauritiaflexuosa Mart) adsorption process by supercritical. The adsorption was carried out experimentally by the method of frontal analysis in columns packed with y-alumina and 15, 20 e 25 MPa, 333 K, and flow of solvent QCO2 = 10,6 L/min, using an assembly of double columns of 81 cm3, tested and approved for use as a cell adsorption. The oil was buriti physic-chemically characterized according to the AOCS official methods and found to comply with the data reported in the literature. The composition of methyl esters was determined by gas chromatography (CG) and antioxidant activity by the method of capture of free radicals (DPPH). The adsorbent was characterized by fluorescence and x-ray diffraction, determining the particle size distribution, porosity and surface area by BET in each experiment, a material balance was performed in the adsorption column to calculate the mass of the adsorbed species in y-alumina in the process of supercritical adsorption. The influence of pressure on supercritical adsorption was investigated by examining the behavior of the isotherms. The Langmuir isotherm was used to model the experimental data of adsorption. The experimental results show an increased capacity adsorbent with higher pressures, showing a maximum of 90,9 ± 8,6 mg oil/g y-alumina, at 25 MPa. The adsorption supercritical buriti oil on γ-alumina using carbon dioxide as solvent appears to be an alternative method for extracting antioxidants from the includ carotenes.
Acesso aberto (Open Access)
Compostos bioativos e potencial antioxidante da fruta Jacaiacá (Antrocaryon amazonicum)
(Universidade Federal do Pará, 2019-05-14) BARBOSA, Anna Paula Pereira; CHISTÉ, Renan Campos
Bioactive compounds are secondary metabolites from plants, which are found in leaves, stem, seeds, peel, flowers and fruits. Such compounds exhibit antioxidant properties, since they can scavenge reactive oxygen species (ROS) and reactive nitrogen species (RNS) as one of the mains mechanisms of action. Among the fruits present in the Amazonian estuary, the Jacaiacá (Antrocaryon amazonicum) was already reported as a promising source of bioactive compounds, such as phenolic compounds and carotenoids. In this study, the individual profiles of carotenoids and phenolic compounds in the pulp and peel of Jacaiacá, from two different cities in Pará State, Brazil (Cametá and São Caetano de Odivelas) was identified and quantified by LC-MS. Moreover, the antioxidant potential of the fruit extracts, from both parts against ROS and RNS of physiological importance, was assessed. According to the proximate composition, water and carbohydrates were the main constituents from the peel and pulp of the jacaiacá pulp. The highest levels of bioactive compounds were found in the fruits of São Caetano de Odivelas in both parts (peel and pulp) and the phenolic compounds contents of 6555.9 mg EAG/100 g and 2855.89 mg EAG/100 g. In relation to carotenoid profiles, the major compounds were all-trans-lutein, followed by all-trans-zeaxanthin and all-trans β cryptoxanthin; in addition all-trans-β-carotene and 9-cis- were also identified. Ten phenolic compounds were identified, being galoyl catechin, catechin, quercetin glucuronide and gallic acid the major ones. Regarding the antioxidant capacity, the peel extracts were more efficient in scavenging ROS and RNS than the pulp extracts. The peel extract showed higher efficiency against singlet oxygen (1O2) (IC50 = 16.41µg / mL), hypochlorous acid (HOCl) (IC50 = 20.19 µg / mL), peroxynitrite (ONOO-) (IC50 = 37.81µg / mL) and superoxide anion radical (O2 •- ) (IC50 = 47.09µg / mL), while the pulp extract was more efficient against ONOO- , followed by HOCl (IC50 = 29.70 µg / mL), ¹O2 (IC50 = 75.84 µg / mL) and less efficient against O2 •- ( IC50 = 144.07 µg / mL). Therefore, jacaiacá fruit was characterized as a great source of bioactive compounds with antioxidant properties to be used by the food, pharmaceutical and cosmetic industries.
Acesso aberto (Open Access)
Obtenção do óleo e da polpa sem gordura do açaí (euterpe oleracea) por extração supercrítica: caracterização físico-química, estudo da citotoxicidade e vida de prateleira
(Universidade Federal do Pará, 2025-01-06) SIQUEIRA, Letícia Maria Martins; BRASIL, Davi do Socorro Barros; http://lattes.cnpq.br/0931007460545219; https://orcid.org/0000-0002-1461-7306; CARVALHO JÚNIOR, Raul Nunes de; http://lattes.cnpq.br/5544305606838748; https://orcid.org/0000-0002-4433-6580
Açaí is a very important product for the development of the state of Pará, for the bioeconomy and the market has been growing. And currently the majority of the state’s production is just fruit pulp, hence the importance of new products with added value and the need to invest in new sustainable processes for the region’s development. Therefore, the objective of this work was to obtain the oil and pulp without açaí fat by supercritical extraction, determine the main bioactive compounds, evaluate the possible cytotoxicity in MRC-5 and VERO cells in vitro and evaluate the shelf life of the products. The freezedried açaí pulp was subjected to extraction with supercritical CO2, under the conditions 323,15 K at 35 MPa, 333,15 K at 42 MPa and 343,15 K at 49 MPa. The highest yield (51,74 %), carotenoids (277,09 μg/g), DPPH (2,55 μmol TE/g), ABTS (2,60 μmol TE/g) and FRAP (15,25 μm ferrous sulfate/g) from oil and ABTS (644,23 μmol TE/g) from pulp without fat were found at 343,15 K at 49 MPa condition. The highest contents of phenolic compounds (150,20 mg GAE/g d.b.), DPPH (414,99 μmol TE/g d.b.) and FRAP (746,2 μm ferrous sulfate/g) from pulp without fat were found at 323,15 K at 35 MPa condition. The pulp without fat presented high levels of anthocyanins without significant variation. The products did not present cytotoxicity. The half-life of the freeze-dried pulp and the fat-free pulp in the presence of light (Becker) was shorter than that of the packaging materials. The sealed packaging achieved a longer half-life compared to vacuum-sealed packaging for the freeze-dried pulp. However, for the fat-free pulp, the vacuum-sealed packaging showed a longer half-life compared to the sealed one. The results of the stability of açaí oil qualify it as a table oil rather than a frying oil, as it degrades more rapidly at higher temperatures. The results demonstrated that the products have potential applications in the industry.
Acesso aberto (Open Access)
Uso combinado de técnicas não convencionais na recuperação de astaxantina do coproduto gerado no processamento de camarão-rosa (farfantepenaeus subtilis)
(Universidade Federal do Pará, 2021-08-31) SILVA, Aline Kazumi Nakata da; RODRIGUES, Antonio Manoel da Cruz; http://lattes.cnpq.br/7524720020580309
The large amount of co-products generated in the production chain of shrimp processing has created a discussion about their use for the manufacture of high-added value ingredients. Regarding this subject, the use is both an attempt to minimize the pollution caused by the inadequate disposal of industrial waste in nature, and a way to generate income through the extraction of substances of economic interest. For this, knowing the amount of carotenoids and polyunsaturated fatty acids of the pink shrimp (Farfantepenaeus subtilis), which is the main species of marine shrimp produced by capture fisheries in the state of Pará, is essential since it is recognized as a source of these substances by the specialized literature. In the present work, two ways of using the pink shrimp co-product were studied, the first through drying in a spouted fluidized bed and the second through the extraction of the astaxanthin (ASX) carotenoid through enzymatic hydrolysis combined with ultrasound-assisted extraction (UAE). For drying the co-product, the spouted bed is a good alternative due to its low operating cost and high efficiency in removing moisture from the material. The co-product (pink shrimp cephalothorax) was ground in a food processor and then dehydrated at temperatures of 70, 80 and 90 °C, and later, conducted to an ASX extraction step using palm olein as a solvent at temperatures of 50, 60 and 70 °C. In carrying out the ASX extraction with enzymatic hydrolysis and UAE, the effects of the enzyme type (alcalase, flavourzyme and a mixture of alcalase and flavourzyme in a ratio of 1:1, v/v), enzyme concentration (0.2; 0.4; 0.6 and 0.8 % relative to the sample mass) and hydrolysis time (0, 60, 90 and 120 minutes). Alcalase was the most efficient enzyme, whose action culminated in the extraction of almost 70 % of the relative content of ASX of the sample. The ASX content was significantly and positively affected by the enzyme concentration (p<0.05) for all the enzymes, but the hydrolysis time was only partially significant.
Acesso aberto (Open Access)
Uso de processo enzimático usando a obtenção de carotenoides da torta residual de dendê
(Universidade Federal do Pará, 2017-02-22) MESQUITA, Eric César Mano; CORRÊA, Nádia Cristina Fernandes; http://lattes.cnpq.br/5763999772352165
The residual fibers from the production of palm oil are a potential source of carotenoids of great economic importance, particularly β-carotene, due to its biological characteristics in human health and its pro-vitamin A. The enzymes cellulite have been shown to be a strong alternative to increase the yield in the oil extraction process, used particularly for the pretreatment of the constituent material of the plants, as it favors the release of the oil inside the plant cell, contributing to the increase of the amount of oil to be extracted and still reducing the extraction time, thus providing the efficiency of the extraction processes of compounds of industrial interest. In the pressed mesocarp pie (TMD), a pre-treatment with the enzymes cellulase (CELLUCLAST) and pectinase (PECTINEX) at the times of 3.6, 12, 24, 48 and 60 h was carried out to evaluate the highest volume yield carotenoid degradation. Then, the effect of the addition of the lipase enzyme was evaluated through a rotational central composite experimental design 2³, on the quality of the oil, in terms of β-carotenes (μg /gTMD48). The results for the pre-treated TMD indicate that 6 hours would be enough to reach this goal, obtaining 55% mass of carotenoids. For the results with addition of lipase, it was observed that in the highest concentration there was a decrease in acidity and an increase in the content of carotenoids. In the present study it was observed that hydrolysis did not occur as a function of the amount of water in the reaction medium favoring the opposite reaction, the esterification of the free fatty acids.