Navegando por Assunto "Carrapato"
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Item Acesso aberto (Open Access) Investigação de Leishmania SP em carrapatos de cães de bairros de Imperatriz-MA, através da Reação em Cadeia da Polimerase (PCR)(Universidade Federal do Pará, 2012) FREITAS, Milena Sousa; ISHIKAWA, Edna Aoba Yassui; http://lattes.cnpq.br/3074963539505872Leishmaniasis is a disease caused by the protozoan Leishmania, can affect humans and animals depending on the species of the parasite, transmitted by sandflies are female insects of the genus Lutzomyia, that in exercising hematophagism inoculated infective promastigote forms, but recently has been raised hypotheses about the transmission by ticks. According to the epidemiological surveillance of Imperatriz-MA is a city endemic for both Cutaneous Leishmaniasis (LT) and for Visceral Leishmaniasis (VL). This study aimed to investigate the presence of Leishmania spp in ticks collected from dogs presented to petshop and Zoonoses Control Centre of the Municipality of Imperatriz using the PCR technique. DNA was extracted from 640 female ticks and tested using the primer which amplifies the mini-exon gene of Leishmania sp. Ticks were collected from 41 dogs of different neighborhoods of Imperatriz. Most ticks were identified as Rhipicephalus sanguineus. The following clinical signs of leishmaniasis in dogs were observed: onychogryphosis in 53.65% (22/41); ulcers 63.41% (26/41), hair loss and loss of appetite in 39.02% (16 / 41). One hundred and seventy ticks (26.56%) of 16 dogs had collected DNA from Leishmania subgenus Viannia, responsible for the cutaneous form of the disease. DNA was not detected none of Leishmania infantum chagasi. Infected ticks were collected from both symptomatic and asymptomatic dogs. Although it has not been shown that ticks can transmit Leishmania to dogs under natural conditions, the outcome of this study has several important because it is a non-invasive method of detection, able to differentiate groups of parasites in circulation, particularly if animals do not have lesions, may be a biological indicator in places where there is an investigation done serological and entomological not and can support the programs of the local health surveillance.Item Acesso aberto (Open Access) Potencial acaricida do óleo de andiroba Carapa guianensis Aubl. sobre fêmeas adultas ingurgitadas de Anocentor nitens Neumann, 1897 e Rhipicephalus sanguineus Latreille, 1806(2009-08) FARIAS, Márcia Paula Oliveira; SOUSA, Dailene Pontes de; ARRUDA, Alberto Cardoso; WANDERLEY, Almir Gonçalves; TEIXEIRA, Whaubtyfran Cabral; ALVES, Leucio Camara; FAUSTINO, Maria Aparecida da GlóriaIn vitro acaricide potential of the oil from andiroba seed (Carapa guianensis) was evaluated on engorged females of Anocentor nitens (n=210) and Rhipicephalus sanguineus (n=140) manually collected, from horses and dogs naturally infested, respectively. Five dilutions, 100%, 50%, 30%, 25%, and 10% of andiroba seed oil in deionized water, using tween 80 as dispersant, were employed for the engorged females immersion test. For A. nitens test, three repetitions were made with each dilution, using 10 engorged females for each treatment, and two repetitions for R. sanguineus test. Two control groups were used for each tick species, one with deionized water and another one with tween 80 and deionized water. After the tests, the females were kept in the laboratory under room temperature. Engorged female mortality and oviposition reduction were observed with infertile eggs, showing 100% of efficacy in the two species in all tested dilutions. The obtained data demonstrated the potential use of andiroba seed extract against A. nitens and R. sanguineus.