Navegando por Assunto "Eleutherine plicata"

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Acesso aberto (Open Access)
Atividade antibacteriana de plantas medicinais frente á bactérias multirresistentes e a sua interação com drogas antimicrobianas
(Universidade Federal do Pará, 2012-08-28) SARAIVA, Rosa Márcia Corrêa; VIEIRA, José Maria dos Santos; http://lattes.cnpq.br/6807452375674442
Infection control of the multidrug-resistant microorganisms sometimes is ineffective even with the development of new antibiotics. Many herbal extracts have antimicrobial effects and may represent an alternative therapy for infectious diseases, mainly when associated with antibiotics of clinical use. The aim of this study was to evaluate the antibacterial activity of medicinal plants in multidrug-resistant microorganisms and their interaction with antimicrobial agents. We evaluate the antibacterial activity of plant extracts and fractions of Eleutherine plicata (“marupazinho”) Geissospermum vellosii (“pau-pereira”) and Portulaca pilosa (“amor-crescido”) against isolates of Oxacillin-Resistant Staphylococcus aureus (ORSA) and multi-resistant bacteria Pseudomonas aeruginosa, from human clinical isolates. Also we evaluate interaction of these plant extracts with antimicrobial agents of clinical use. The antibacterial activity was determined by disk diffusion on Mueller Hinton agar and the Minimum Inhibitory Concentration (MIC) by micro dilution plate technique using Muller Hinton broth as culture medium and 0.01% resazurin as a developer of bacterial growth. The extracts and fractions were tested at concentrations of 500, 250, 125, 62.5, 31.2 and 16.2 μg/mL dissolved in 10% DMSO. Plants E. plicata and G. vellosii demonstrated activity against ORSA isolates with MICs of 125 μg/mL, whereas P. pilosa had an effect on the isolates of P. aeruginosa with MIC of 250 μg/mL. There were 25% of synergism and only 5% of antagonism of all 120 plant and antimicrobial agents interaction tested. ORSA isolates had synergistic interaction with ciprofloxacin, clindamycin and vancomycin agents and with both plant derivatives of E. plicata and G. vellosii. The derivatives of P. pilosa potentiated the action of the aztreonam, cefepime and piperacillin + tazobactam agents compared to the isolates of P. aeruginosa multidrug-resistant. The results shows therapeutic potential of E. plicata, G. vellosii and P. pilosa in the control of bacterial infections involving multidrug-resistant phenotype (MDR) and its interaction with antibacterial agents may represent a new alternative in the therapy of these infections.
Acesso aberto (Open Access)
Estudos de citotoxicidade e genotoxicidade de Eleutherine plicata Herb
(Universidade Federal do Pará, 2014-09-30) GALUCIO, Natasha Costa da Rocha; BAHIA, Marcelo de Oliveira; http://lattes.cnpq.br/3219037174956649; DOLABELA, Maria Fâni; http://lattes.cnpq.br/0458080121943649
The purpose of this study was phytochemical studies of E. plicata, and to evaluate the cytotoxicity, the role of oxidative stress and genotoxicity. The powder of E. plicata bulbs underwent maceration with ethanol, the solution concentrated to residue in rotaevaporator. The ethanol extract was subjected to fractionation by open column chromatography over silica gel, being used as the mobile phase solvents of increasing polarity. The dichloromethane fraction was subjected to fractionation by preparative layer chromatography using dichloromethane as mobile phase, and 3 subfractions obtained. The ethanol extract, fractions and subfractions were subjected to chromatographic and spectrophotometric analysis. All samples were subjected to the tests: cellular viability (MTT), the antioxidant capacity (DPPH), comet and micronucleus assays. From the ethanol extract obtained a rich fraction naphthoquinone (dichloromethane fraction). Fractionation of this led to the isolation of: S1, S2 (major fraction), and fraction of minority S3 (unidentified, not tested). Chromatographic studies and spectrophotometric allowed the identification of S2 (isoeleuterin). Fractionation contributed positively to cytotoxicity on VERO cells, the sample being more cytotoxic to S1. The cytotoxicity in HepG2 cells was concentration dependent, being the fractionation did not contribute positively to this. Also, over time, the longer the exposure time, the lower the cytotoxicity to HepG2 cells. The maximum antioxidant activity was observed for subfraction S1, and this low genotoxicity possessed by both methods and it was the most cytotoxic. The dichloromethane fraction has an intermediate antioxidant capacity, but had a high genotoxicity in micronucleus assay. The isoeleuterin (S2) was lower antioxidant capacity, lower cytotoxicity and genotoxicity conflicting results. The ethanol extract possessed the lowest antioxidant capacity, moderate genotoxicity and lower cytotoxicity. When analyzing the results occur that: a subfraction S1 is the most promising candidate as the antimalarial drug, as have cytotoxicity and genotoxicity rates at acceptable levels. The isoeleuterin needs additional research on 11 genotoxicity. Regarding the dichloromethane fraction was not advisable to use for the development of an antimalarial drug, since it is more genotoxic.
Acesso aberto (Open Access)
Estudos químico-farmacêuticos, atividade antitumoral e mutagênica de Eleutherine plicata. Herb.
(Universidade Federal do Pará, 2020-11-03) CASTRO, Ana Laura Gadelha; MONTEIRO, Marta Chagas; http://lattes.cnpq.br/6710783324317390; https://orcid.org/ 0000-0002-3328-5650; DOLABELA, Maria Fâni; http://lattes.cnpq.br/0458080121943649; https://orcid.org/ 0000-0003-0804-5804
Naphthoquinones have been linked to antitumor activity; however, they can cause DNA damage and be mutagenic. Some of these as the isoeleutherine and eleutherine, present in Eleutherine plicata Herb., do not have studies of mutagenicity and antitumor activity. This study evaluated the antitumor potential of the ethanolic extracts of E. plicata (EEEP), the fraction in which the quinones were found (Fraction dichloromethane-FDEP) and the genotoxic potential related to these substances. For understanding whether fractionation influences antitumor activity, EEEP was obtained by macerating the bulbs, it was fractionated under reflux and FDEP was obtained and fractionated in an open chromatographic column, resulting in the isolatation of eleutherine, isoeleutherine and eleutherol. Chemical-pharmaceutical studies were carried out: silica toxicity and pharmacokinetics (PreADMET), docking and dynamics. The antitumor activity was evaluated through the cytotoxicity in tumor cell line (oral cancer-SCC-9) and normal cell line (human keratinocytes-HaCaT), three-dimensional assay in spheroid model and cell migration in the same lines. Genotoxicity and mutagenicity were evaluated in Allium cepa model, the results of the isolated compounds were similar to those obtained in silico studies. In addition to the isoeleutherine and eleutherine, eleuterol was isolated from FDEP, the silica evaluation demonstrated similar pharmacokinetic profiles between these compounds. In the evaluation of anti-tumor activities, the EEEP fractionation contributed negatively to the activity, with EEEP (SCC09: IC50 = 12.87 ± 0.86 and HaCaT: 28.81 ± 1.82µg/mL) being the most promising with higher selectivity index for the tumor cell, interfering in the speed and directionality of tumor migration. Trying to understand this result, the capacity of naphthoquinones bind to Topoisomerase II (TOPII) was evaluated, confirming that they bind in its pouch, stabilizing the DNA-TOP II complex. In contrast, eleutherine proved to be more genotoxic, increasing the rate of mitosis, of aberrations, with micronucleus, bud and bridge being observed in the metaphase phase. However, it was not possible to differentiate the toxicity of eleutherine and isoleutherine in silico studies (Algae, Daphinia, fish and mutagenicity), obtaining similar results. In summary, EEEP is promising as a cytotoxic agent in tumor cells, and in the prevention of squamous cancer of the mouth. In relation to naphthoquinones, isoleutherine, due to its lower toxic potential and stabilization capacity of the DNA-TOP II complex, needs to be evaluated in other tumor strains.
Acesso aberto (Open Access)
Isoeleuterol e isoeleuterina: potenciais marcadores químicos da tintura de Eleutherine plicata Herb (Iridaceae) e atividades microbiológica e antioxidante
(Universidade Federal do Pará, 2008-12-30) MALHEIROS, Luiz Claudio da Silva; VIEIRA, José Maria dos Santos; http://lattes.cnpq.br/6807452375674442; BARBOSA, Wagner Luiz Ramos; http://lattes.cnpq.br/1372405563294070
Eleutherine plicata Herb. is an Iridaceae, popularly known as marupazinho, widely used by people to treat diarrhea. With the bulb of the plant is about a cup of tea, which is used to treat infestations caused by amoeba. The plant material used in this study was collected in Belém do Pará and their identification by comparison of botany was deposited in exsiccates Paraense Emilio Goeldi Museum under nº 10543. The ethanol extract was prepared by percolation from the bulb previously dried and crushed. After drying the ethanol extract was suspended in a solution hydroalcoholic (1:1) and subjected to partition with solvents of increasing polarity. With the ethanol extract and the fractions were performed eighteen tests to detect classes of secondary metabolites. The ethanol extract and the fractions hexanic and chloroform, showed a positive result for naphthoquinones, anthraquinones and steroids and triperpenoids. The analysis by thin-layer chromatography fractions of ethanol extract and hexane and chloroform, showed areas susceptible to methanolic solution of KOH 10%, indicating the presence of quinones in these samples. The evaluation of the antimicrobial activity of ethanol extract and fractions hexanic and chloroform with strains of C. albicans, S. aureus, E. coli and P. aeruginosa, showed that the chloroform fraction is the most active, presenting the largest halos of inhibition of microbial growth, possibly containing a higher concentration of active constituents. The chemical constituents isoeleutherol and isoeleutherine were isolated from chloroform fraction, and were characterized chemically using RMN 1H e 13C, compared with the literature. The ethanol extract, isoeleutherol and isoeleutherine were submitted to evaluation of their antioxidant activies, and showed weak activity when compared with BHT.