Navegando por Assunto "Neoplasias da mama"

Agora exibindo 1 - 9 de 9

Acesso aberto (Open Access)
Associação do perfil de acetilação lenta do gene NAT2 na susceptibilidade ao câncer, na Região Norte do Brasil
(Universidade Federal do Pará, 2013-04-10) FERNANDES, Marianne Rodrigues; SANTOS, Ney Pereira Carneiro dos; http://lattes.cnpq.br/1290427033107137; BURBANO, Rommel Mario Rodriguéz; http://lattes.cnpq.br/4362051219348099
Objectives: The N-acetyltransferase 2 (NAT2) gene is a marker for the study of interindividual susceptibility to develop malignant neoplasms, once the enzyme NAT2 takes part in the metabolism of carcinogenic agents and the single nucleotide polymorphism (SNP) of its gene produces enzymes with different activities, leading to either slow or fast acetylation of xenobiotics. The purpose of this study was to investigate a possible association between the NAT2 gene SNPS and susceptibility to the involvement of gastric adenocarcinoma or invasive ductal carcinoma of the breast in patients of northern Brazil. Methods: Five polymorphisms of great importance for defining the metabolism profile of enzyme NAT2 (C282T, T341C, C481T, A803G and G857A) were investigated by direct sequencing of 986 base pairs, amplified in two PCR reactions, totalizing 133 patients with neoplasms (63 with Gastric Cancer-GC and 70 with Breast Cancer-BC) and 89 Control subjects. In order to avoid spurious interpretations resulting from the population substructure, we used a panei with 48 ancestry informative markers (AIM). Results: We found statistical differences for African and European parental contribution when compared between the Cancer and Control groups; a higher African contribution was detected in the study group with Cancer and, in the control group, it was detected a higher European contribution (p<0.001). Dominating polymorph genotypes C282T (TT + CT) showed significant association (p<0.001; OR 3.076; Cl 95% 1.664-5.687) for susceptibility to the different forms of Cancer investigated. A significant association of slow and fast acetylation profile with the susceptibility to develop the investigated neoplasms was noticed (p=0.010; OR 3.054; Cl 95% 1.303-7.159) and (p= 0.041; OR 0.527 Cl 95% 0.280-0.973) clearly showing that individuais with slow acetylator profile showed a risk of developing neoplasms increased to up to three times when compared to Control subjects. Conclusions: Ancestry genomic control was effectively important for this investigation and enabled the control of the ancestry effect on the association of NAT2 gene for susceptibility to cancer. In this study, it was possible to prove the strong influence of xenobiotics slow acetylation profile on the susceptibility to GC and BC.
Acesso aberto (Open Access)
Cytogenetic description of breast fibroadenomas: alterations related solely to proliferation?
(2001-08) BURBANO, Rommel Mario Rodriguéz; LIMA, Eleonidas Moura; KHAYAT, André Salim; BARBIERI Neto, J.; CABRAL, Isabel Rosa; BASTOS JR., L.; BAHIA, Marcelo de Oliveira; CASARTELLI, Cacilda
Twelve breast fibroadenomas were analyzed cytogenetically and only four were found to have clonal alterations. The presence of chromosomal alterations in fibroadenomas must be the consequence of the proliferating process and must not be related to the etiology of this type of lesion. In contrast, the few fibroadenomas that exhibit chromosomal alterations are likely to be those presenting a risk of neoplastic transformation. Clonal numerical alterations involved chromosomes 8, 18, 19, and 21. Of the chromosomal alterations found in the present study, only monosomy of chromosomes 19 and 21 has been reported in breast fibroadenomas. The loss of chromosome 21 was the most frequent alteration found in our sample. The study of benign proliferations and their comparison with chromosome alterations in their malignant counterparts ought to result in a better understanding of the genes acting on cell proliferation alone, and of the genes that cause these cells to exhibit varied behaviors such as recurrences, spontaneous regression and fast growth.
Acesso aberto (Open Access)
Detecção molecular do Papilomavirus humano (HPV) em amostras teciduais de tumores da mama
(Universidade Federal do Pará, 2010) ROCHA, Francianne Silva; QUARESMA, Juarez Antônio Simões; http://lattes.cnpq.br/3350166863853054
The malignant neoplasm of breast is a major cause of female mortality, considered as a public health problem. In this work researched the presence of human papillomavirus (HPV) in benign and malignant breast tumors and normal breast tissue samples. Was used the technique of Polymerase chain reaction (PCR) for HPV DNA molecular detection in 63 patients, thus distributed: 28 malignant and 17 benign tumors and 18 are samples of normal breast. Our results showed positivity for HPV DNA sequence in 11 samples, all belonging to the bearers of malignant tumors: 17.4% of all samples and 39.2% of malignant tumors. All positive HPV DNA showed tumors for oncogênicos types 16 and/or 18, was not detected HPV 6 and 11 DNA. The results demonstrated high positivity to the hormonal receptors in positive samples examined and presented a follow-up with prevalence of adverse events as relapse loco-regional metastases and death in with HPV DNA. Finds ratify the data found in the literature, showing a possible participation of this virus in the development of breast cancer and possible unfavorable contribution in clinical evolution.
Acesso aberto (Open Access)
Expressão dos genes C-MYC, HER-2 e receptores hormonais como preditores de resposta à quimioterapia neoadjuvante em câncer mamário
(Universidade Federal do Pará, 2010-08-02) PEREIRA, Cynthia Mara Brito Lins; DEMACHKI, Samia; http://lattes.cnpq.br/7568391537270652; BURBANO, Rommel Mario Rodriguéz; http://lattes.cnpq.br/4362051219348099
Breast cancer is a higher incidence of tumors in women, and therefore many studies have been performed since the assessment of the epidemiological characteristics, the dynamics biocelular and treatment of this disease. In evaluating responses to treatment, the risk factors are markers that help in choosing the best drug to use. This work aimed to evaluate the genes of estrogen and progesterone receptors, HER-2 and C-MYC in locally advanced breast tumors as predictors of response to neoadjuvant chemotherapy. We studied fragments of mammary malignancy in 50 patients with infiltrating ductal carcinoma, with clinical stage III and E-treated with neoadjuvant chemotherapy. We used the techniques of immunohistochemistry and fluorescence in situ hybridization (FISH). The analysis of hormone receptors showed no statistically significant difference comparing patients with satisfactory response to chemotherapy, the poor and the analysis of HER-2 showed significance only for satisfactory answers, where there was poor amplification of this gene. Regarding C-MYC was observed a statistically significant difference comparing the high amplification of this gene to a poor response to chemotherapy. The study concluded that the C-MYC gene may be an important marker for predicting the treatments used in neoadjuvant chemotherapy for breast cancer.
Acesso aberto (Open Access)
Fatores associados à qualidade de vida de mulheres submetidas à radioterapia
(Universidade Federal do Rio Grande do Sul, 2024) VILHENA, Fabiane Diniz Machado; PEREIRA, Odenilce Vieira; SOUSA, Fabianne de Jesus Dias de; MARTINS, Nandara Celana Negreiros; ALBUQUERQUE, Gisela Pereira Xavier; LOPES, Renata Glaucia Barros da Silva; SAGICA, Taís dos Passos; RAMOS, Aline Maria Pereira Cruz
Objective: To evaluate the skin characteristics and quality of life of patients with breast cancer undergoing radiotherapy. Method: Cross-sectional study conducted with 60 women. The classification scales of skin changes resulting from exposure to ionizing radiation (RTOG) and the validated versions in Portuguese of those that classified skin types (Fitzpatrick), symptoms (RISRAS) and quality of life (DLQI) were applied. in the period between December 2021 and October 2022. For data analysis, Fisher’s Exact Test, Chi-Square and Asymptotic General Independence Test were used. Results:100% of patients had skin irritation. As the treatment progressed and the radiodermatitis appeared or worsened, there was a tendency for the intensity of signs and symptoms to increase, such as: sensitivity, discomfort or pain, itching, burning and heat, dry and wet desquamation, which may have impacted the quality of life and reflected in other aspects, such as: shopping activities or outings (p=0.0020), social activities or leisure activities (p=0.0420). Conclusion: Radiodermatitis is a common condition that affects women with breast cancer undergoing radiotherapy, skin characteristics and quality of life of patients affected during this treatment
Acesso aberto (Open Access)
Impacto da pandemia da COVID-19 no rastreamento, diagnóstico e tratamento do câncer de mama em mulheres a partir de 50 anos, no estado do Pará
(Universidade Federal do Pará, 2023-08-10) DIAS, Paula Danniele dos Santos; SANTANA, Mary Elizabeth de; http://lattes.cnpq.br/6616236152960399; https://orcid.org/0000-0002-3629-8932
Breast cancer is the second most common in the world and the most common among women. Early diagnosis and treatment estimate a good prognosis for the disease. After the pandemic was declared by the World Health Organization (WHO), States had to adopt restrictive measures to contain the spread of the disease. Brazil was one of the four countries with the highest number of confirmed Covid-19 infections, with high transmissibility rates causing a collapse in health services. As a result, the maintenance and regular treatment of several diseases, including breast cancer, were affected. Objective: to analyze whether the Covid-19 pandemic affected the screening, diagnosis and treatment of breast cancer, in women aged 50 and over, in the State of Pará. Method: The study was retrospective, cross-sectional, with a quantitative approach, using secondary data obtained from the Information Technology Department of the Unified Health System (DATASUS). An analysis was carried out of the number of exams carried out in the post-pandemic (2018-2019) and pandemic (2020-2021) period based on the percentage variation, and application of the chi-square test and G test for the time taken to perform exams and time to start treatment. Results: During the pandemic period, a greater number of screening mammograms (+3.68%), cytological (+23.68%), histological (+10.7%) and a lower number of diagnostic mammograms (-38.7%) were observed %). The time interval for carrying out the exams was up to 30 days for screening and diagnostic exams during the pandemic period. The observed time to start treatment was greater than 60 days, with a greater number of cases treated during the pandemic period. Conclusion: the study points out that statistically the Covid-19 pandemic did not interfere with the screening, diagnosis and treatment of breast cancer, in women over 50 years old, in the State of Pará. The effects of Covid-19 will only possibly be observed at long-term, therefore, studies should be carried out that permeate the development of strategies that prepare health services for a future state of health emergency, to mitigate greater impacts on the health of the population.
Acesso aberto (Open Access)
Investigação de polimorfismos nos genes XRCC1, MTHFR e EGFR como possíveis marcadores de suscetibilidade ao câncer, na população de Belém-PA
(Universidade Federal do Pará, 2013-04-08) VIEIRA, Priscilla Cristina Moura; BURBANO, Rommel Mario Rodriguéz; SANTOS, Ney Pereira Carneiro dos; http://lattes.cnpq.br/4362051219348099; http://lattes.cnpq.br/1290427033107137
Cancer is defined as a multifactorial disease resulting from complex interactions between extrinsic and intrinsic factors. Among the main intrinsic factors are the genetic and/or epigenetic alterations in genes involved with the carcinogenesis process. The identification and characterization of these genes may provide a better understanding of the molecular basis of cancer. Considering the importance of alterations in XRCC1, MRHFR and EGFR genes in various pro-carcinogenic pathways, it is extremely important to investigate the effects of functional polymorphisms in these genes and their molecular consequences in cancer susceptibility.The objective of this study was to identify possible associations between single nucleotide polymorphisms (SNPs) Arg194Trp (XRCC1) e Ala222Val (MTHFR) e Arg521Lys (EGFR) with the development of gastric and breast cancers in the population of Belém-PA, in a case-control study. Furthermore, the control of genomic ancestry was held to avoid spurious results arising from population substructuring in the groups investigated. Molecular analysis of SNPs was carried out by TaqMan. Statistical analyses were performed using the program SPSS v.20 and to estimate the interethnic admixture we used the program STRUCTURE v.2.2. Regarding polymorphisms Arg194Trp, Ala222Val we did not observe any significant association with susceptibility to breast and gastric tumors (P > 0.05).For the polymorphism Arg521Lys, in a first moment (univariate analysis), a significant effect for susceptibility to cancers investigated was found (P = 0.037). However, after genomic control for African and European ancestries, this result has proved to be spurious (P = 0.064). Regarding ancestries, our results showed a strong association of African ancestry with susceptibility to gastric and breast cancers (P = 0.010, OR = 76,723; 95% CI = 2.805 - 2098.230) whereas for European contribution a protective effect was found (P = 0.024, OR = 0071, 95% CI = 0.007-0.703). In conclusion, our study presented the evidence that the African and European genomic ancestries are important factors related to susceptibility to gastric and breast cancers. Regarding Arg521Ly polymorphism, further studies are necessary to confirm whether the association is indeed spurious.
Acesso aberto (Open Access)
Investigation of single-strand conformational polymorphism of the TP53 gene in women with a family history of breast cancer
(2000-11) BURBANO, Rommel Mario Rodriguéz; MEDEIROS, Arnaldo Correia de; MELLO, Adriano Azevedo de; LEMOS, José Alexandre Rodrigues de; BAHIA, Marcelo de Oliveira; CASARTELLI, Cacilda
Breast cancer in families with germ line mutations in the TP53 gene has been described in the medical literature. Mutation screening for susceptibility genes should allow effective prophylactic and preventive measures. Using single-strand conformational polymorphism, we screened for mutations in exons 5, 6, 7 and 8 of gene TP53 in the peripheral blood of 8 young non-affected members (17 to 36 years old) of families with a history of breast cancer. Studies of this type on young patients (mean age, 25 years) are very rare in the literature. The identification of these mutations would contribute to genetic counseling of members of families with predisposition to breast cancer. The results obtained did not show any polymorphism indicating mutation. In our sample, the familial tumorigenesis is probably related to other gene etiologies.
Acesso aberto (Open Access)
Parâmetros de saúde metabólica e visual em pacientes diagnosticadas com câncer de mama em tratamento anticâncer
(Universidade Federal do Pará, 2021-09) SIQUEIRA, Maria Lúcia Souza; SOUZA, Givago da Silva; http://lattes.cnpq.br/5705421011644718; https://orcid.org/0000-0002-4525-3971; MONTEIRO, Marta Chagas; http://lattes.cnpq.br/6710783324317390; https://orcid.org/0000-0002-3328-5650
Female breast cancer, according to current estimates, is still reaching a very high level of incidence and prevalence in Brazil and Pará. The need for studies that expand knowledge about therapeutic options and mitigate the damage suffered by cancer becomes innovative in our region. Thus, we aim to determine and assess metabolic and visual health parameters in patients diagnosed with breast cancer exposed to anti-cancer therapies. The specific objectives were divided into three sections: Section I: determine lipid, anthropometric, oxidative stress and pro-inflammatory cytokine markers to assess metabolic health; Section II: determine tamoxifen metabolites (4-hydroxy tamoxifen and endoxifen) and correlate with blood lipids to assess drug interaction and metabolism with lipids; Section III: Perform visual tests to assess the effect of anticancer therapies on retinal thickness. Research approved by the ethics committee with opinion number 1.915.051 (CEP-HOL) and 1.897.057 (CEP-ICS-UFPA) from March 2017 to September 2019. It consisted of 40 women diagnosed with breast cancer (Ductal Carcinoma Grade II and III) in a public cancer reference hospital in Belém of Pará and who consented to participate in the research. A group of 20 patients formed the chemotherapy group (GQt), 20 patients the tamoxifen hormone therapy group (GTam) and another group of women without cancer was constituted as a comparative control (GC) based on the inclusion criteria. For the determination of biochemical markers, the automated method was used; anthropometry followed the Ministry of Health manual; for oxidative stress the colorimetric method; for the measurement of cytokines the ELISA method; for the determination of tamoxifen and metabolites in plasma, High Performance Liquid Chromatography (HPLC) was used; for retinal analyses, optical coherence tomography (OCT) was used. The results obtained showed that the patients were predominantly women aged between 40 and 50 years, brown, with an income of 1 to 3 minimum wages, were in pre- and post-menopause; were overweight/obese with high BMI, between 25 to 29.9 kg/m2, waist circumference above 80 cm and did not perform regular physical activity; the mean levels of lipids were altered in both groups studied, but with emphasis on the chemotherapy group with elevation of total cholesterol, LDL and triglycerides and low levels of HDL cholesterol, (p<0.05); the antioxidant parameters (TEAC, GSH, CAT and SOD) showed low levels of GSH and high CAT activity for the chemotherapy group and less activity for the tamoxifen group compared to the control (p<0.01); the pro-oxidant parameters (MDA and NO) showed that chemotherapy patients had a higher level of lipid peroxidation than tamoxifen patients compared to control (p<0.05); the GSH/MDA ratio showed greater sensitivity to oxidative damage for chemotherapy patients (p<0.01); as for cytokines, TNF-α and IL-6, they were elevated both in the chemotherapy group and in the tamoxifen group (p<0.05). Mean plasma concentrations of tamoxifen, 4-hydroxy tamoxifen, and endoxifen were 62 ng/mL, 1.04 ng/mL and 8.79 ng/mL; triglyceride levels ranged from 59 to 352 mg/dL, total cholesterol from 157 to 321 mg/dL, LDL-c from 72 mg/dL to 176 mg/dL and HDL-c from 25.1 mg/dL to 62.8 mg/dL; there was no significant association between tamoxifen and metabolites with cholesterol and triglyceride levels; there was a weak association between tamoxifen and its active metabolites with HDLc, LDLc and VLDLc. Mean retinal macular thickness revealed no significant difference between patients who received chemoradiotherapy and control tamoxifen (p>0.05); regional macular thickness revealed that only one macular field showed a significant difference between two groups; in the external nasal macular field, the chemoradiotherapy patients showed thinner retinal thickness compared to the control. We conclude that breast cancer patients exposed to chemotherapy and hormone therapy presented: unfavorable lipid profile with high levels of total cholesterol, LDL, triglycerides and low HDL, overweight and obesity, lower antioxidant capacity for patients who received chemotherapy and systemic levels of inflammatory cytokines, TNF-α and IL-6, elevated; there was a weak association between plasma concentrations of tamoxifen and its active metabolites with levels of HDL-c, LDL-c and VLDL-c, with a low impact of lipoprotein levels on exposure to tamoxifen, 4-hydroxytamoxifen and endoxifen; a macular field was altered in the chemotherapy group, which had a thinner retina compared to the control group, however the retinal structure was sensitive to the presence of tamoxifen metabolites.