Navegando por Assunto "Proteína concentrada"
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Item Acesso aberto (Open Access) Funcionalidades de peptídeos provenientes de biomassas de oleaginosas amazônicas(Universidade Federal do Pará, 2025-04-22) PANTOJA, Gabriela Vieira; FONTANARI, Gustavo Guadagnucci; http://lattes.cnpq.br/6654478241936919; HTTPS://ORCID.ORG/0000-0002-8417-263X; MARTINS, Luiza Helena da Silva; http://lattes.cnpq.br/1164249317889517; https://orcid.org/0000-0003-1911-4502The use of agro-industrial by-products has been increasingly emphasized, as it focuses on sustainability and resource scarcity issues. Moreover, industrial processing by-products may also contain bioactive molecules. Bioactive peptides are molecules that exhibit a wide range of functionalities, including antimicrobial and antioxidant activities. Therefore, the aim of this study was to obtain protein hydrolysates with antioxidant and antimicrobial activity from the residual cakes of Amazonian biomasses tucumã (Astrocaryum vulgare), murumuru (Astrocaryum murumuru), and andiroba (Carapa guianensis) through enzymatic hydrolysis. To achieve this, the protein extraction was first optimized, and once the conditions of pH, ratio, and extraction time were defined, concentrated proteins were obtained and subsequently subjected to enzymatic hydrolysis using trypsin and alcalase. These hydrolysates were tested for phenolic compounds, antioxidant activity, antimicrobial activity, and separated by highperformance liquid chromatography (HPLC). The results of the study were positive regarding antioxidant activity, with tucumã hydrolysates showing the highest content. The andirobaalcalase and tucumã-trypsin hydrolysates exhibited moderate microbial inhibition activity, with this result tending, according to Pearson correlation, to be due to the peptide fractions obtained. Thus, it was possible to conclude that the use of residual oilseed cakes from the Amazon for the extraction of bioactive peptides is a viable practice for generating high-value by-products.