Navegando por Assunto "Proteômica"

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Acesso aberto (Open Access)
A exposição prolongada ao fluoreto durante a adolescência à fase adulta modula o proteoma hipocampal e gera danos cognitivos associados a um padrão neurodegenerativo no hipocampo de camundongos
(Universidade Federal do Pará, 2021-11-19) BITTENCOURT, Leonardo de Oliveira; GOMES, Bruna Puty Silva; http://lattes.cnpq.br/3013433540155834; https://orcid.org/ 0000-0001-5570-8530; LIMA, Rafael Rodrigues; http://lattes.cnpq.br/3512648574555468; https://orcid.org/ 0000-0003-1486-4013
Fluoride (F) is used in artificial water fluoridation due to its anticariogenic activity, but it is also present in soils and natural reservoirs due natural high levels or anthropogenic actions, featuring it as a potential environment toxicant. In addition to skeletal and dental fluorosis in people living in regions with high F levels, some studies have explored the association between F exposure and cognitive damages, and although the evidences have indicated that only high levels pose a deleterious effect on cognition, a heated debated has grown regarding the safety of water supply artificial fluoridation. In this way, this study investigated whether long-term F exposure, from adolescence to adulthood, under the paradigms of water fluoridation and environmental issue, would be associated with memory and learning impairments in mice, and unravel molecular and morphological aspects involved. For this, 21-days-old mice received for 60 days, 10mg/L or 50mg/L of F in drinking water; the control group received only water without addition of F. Then, the cognitive functions were assessed by the Morris Water Maze and Inhibitory Step-down Avoidance test, followed blood and hippocampus collection for plasma F level determination, and hippocampal global proteomic profile analysis by Mass Spectrometry. Some animals were perfused for immunohistochemical analyses of mature neurons density in CA1, CA3, hilus and dentate gyrus (DG) regions. The results indicated that prolonged exposure to F from adolescence to adulthood increased plasma F bioavailability, and the higher F concentration triggered short- and long-term memory impairments, being associated with the modulation of the global proteomic profile, and a neurodegenerative pattern in the CA3 and DG regions. Our data, in a translational perspective, gives evidences of potential molecular targets of F neurotoxicity in the hippocampus and reinforces the safety of low fluoride concentration exposure, besides the need for attention of people living in endemic regions.
Acesso aberto (Open Access)
Extração e análise eletroforética em gel de poliacrilamida (SDS-PAGE) de proteínas totais de folhas e raízes de Piper tuberculatum
(Instituto Nacional de Pesquisas da Amazônia, 2009) SILVA, Redinaldo dos Santos; SOUZA, Claudia Regina Batista de
The Pará State is the principal Brazilian producer of black pepper (Piper nigrum Link), however, the spice’s production has been damaged by the Fusarium disease. The Fusarium solani f. sp. piperis, causal agent of this disease which attacks the plant root system promoting the root rot, leaves fall and plant death. Piperaceae plants endemic of Amazon region and resistant to F. solani f. sp. piperis infection, such as Piper tuberculatum Jacq., which has been studied in order to understand this host-pathogen interaction. In this work were evaluated five conditions for total protein extraction aiming to select suitable buffers for extraction of total proteins from P. tuberculatum leaves and roots. The conditions used to roots and leaves protein extractions were salt buffer, sucrose buffer, glycerol buffer, urea buffer and sodium phosphate buffer. Quantitative analysis showed that sucrose, glycerol and urea buffers were more efficient for leaves and roots protein extractions. SDS-PAGE analysis showed distinct band patterns in leaves and roots protein extracts obtained with different buffers. Our results could support the selection of extraction buffers for proteomics analysis in P. tuberculatum - F. solani f sp. piperis interaction studies.
Acesso aberto (Open Access)
Metaproteômica como ferramenta tecnológica para otimização dos parâmetros de fermentação do milho para formulação de pasta alimentícia
(Universidade Federal do Pará, 2023-06-05) BAHULE, Celina Eugenio; MARTINS, Luiza Helena da Silva; http://lattes.cnpq.br/1164249317889517; LOPES, Alessandra Santos; http://lattes.cnpq.br/8156697119235191
In most developing countries, maize products are often what alleviate hunger and guarantee the livelihood of the population. Fermentation is a processing medium used to soften the corn kernel, improve flavor and digestibility, and some fermented corn products are used in the belief that they have functional properties. Maize dough, obtained by fermentation, has already been reported as having a probiotic and nutraceutical effect, however, other studies discourage its consumption, justifying it by the load and diversity of microorganisms that develop during fermentation. With the aim of studying the functional profile and looking for likely ways to optimize maize dough, a recreation of the spontaneous fermentation of corn was performed, and the formulated dough was subjected to metaproteomics analysis. A total of 53977 peptides were recovered and identified, of which more than 60% were associated with the substrate, 6% with fungi, and 34% with bacteria. Metaproteomics revealed a probable association between Actinobacteria and starch degradation enzymes in the matrix. Proteobacteria (pathogenic majority) and Firmicutes (probiotic part) coexisted and multiplied in equal proportion until the end, allied to this, the high count of mesophiles(213 x 106 CFU/g) and the timid reduction of the pH (4.42) suggested a food product not completely safe. As a way to optimize the process, the maize was autoclaved at 121oC for 20 min for enzymatic inactivation and elimination of microorganisms, and then it was inoculated with Pediococcus acidilati. Untreated maize was also inoculated. The performance of the strain both in pre-treated maize (T+I) and in untreated maize (nT+I) was considered excellent, since, in the first 24 hours, the pH values, 4.2 for nT+I samples and 4.41 for T+I samples were at levels considered safe and decreased with fermentation time. The strong correlation between the fermentation parameters when P.acidilati was used for maize inoculation, opens possibilities for the application of optimization models towards the fast, safe, and industrial production of maize dough. Indeed, metaproteomics, as culture-free and protein-based technique, allows the rapid analysis of the microbiota and the identification of active microorganisms, with the advantage of displaying and correlating the microbial profile with functionality, and are well indicated to study fermented foods quickly and concisely, demonstrating effective methods for optimization