Análise Quantitativa de Neurônio e Interleucina -1 beta em ratos que submetido a Status Epilepticus pelo Modelo de Pilocarpina

Abstract

Epilepsy is the predisposition to develop spontaneo and rec rren epileptic seiz res It is one of the most common diseases of the nervo system in the world pop latio and aro n 30% of patients have Refractory Epilepsy, which is why there is great interest in developing new forms of treatment and st dyin the pathophysiology of the disease in animal models. One of the main refractory epilepsies is Temporal Lobe Epilepsy (TLE), which s al presents a lesion in the hippocampal region called Hippocampal Sclerosis (HS), which is why the hippocamp is one of the most st die epileptic foci. Traditionally, an increase in IL-1 beta expression and microglial activation are characteristic of an inflammatory process in the nervo system. The inflammatory response is a form of defense against local aggression, b when exaggerated it can increase tiss damage, incl din in epilepsy. In this st dy to identify and q antif the tendency of ne rona loss and inflammatory process in the hippocamp after Stat epileptic ind ce by pilocarpine, 11 s rvivin animals were divided into 3 gro ps Control gro (n=5), only saline sol tio witho being ind ce to epileptic seiz res with a s rviva time of 7 days; Pilocarpine gro 1 day (n=3), animals s rvivin to 1 day after seiz r ind ction Pilocarpine 7-day gro (n=3), animals s rvivin to 7 days after seiz r ind ction Then, the brains were extracted and imm nohistochemica testing was performed with anti-Ne and anti-IL-1beta antibodies to co n cells in the marking pattern. Every effort was made to avoid excessive stress to the animals d rin the proced res The morphology of the hippocamp and the labeling of cells with the Anti-Ne antibody was observed in the three gro ps the control gro presented more cells than the others, Anti-IL-1beta marking was weaker in the control gro p and red ce in the 7-day pilocarpine gro p and with greater intensity and expression in the 1-day pilocarpine gro p the gro p were compared sin the Kr skal-Walli statistical test and did not show significant differences, b a pattern was observed in accordance with that predicted in a previo st d that can be investigated with other samples abo the development of the inflammatory process and its migration to the different hippocampal areas and we reaffirm the relevance of prevention and early treatment of crises.