Dissertações em Genética e Biologia Molecular (Mestrado) - PPGBM/ICB
URI Permanente para esta coleçãohttps://repositorio.ufpa.br/handle/2011/10330
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Item Acesso aberto (Open Access) Obtenção de genomas bacterianos a partir de dados metagenômicos da usina hidrelétrica de Tucuruí(Universidade Federal do Pará, 2023-09) PADILHA, Marcos Daniel Mendes; GRAÇAS, Diego Assis das; http://lattes.cnpq.br/1945468865634432; https://orcid.org/0000-0002-5059-4316Tucuruí is the second largest Brazilian Hydroelectric Power Plant, the artificial lake in the municipality of Tucuruí has altered the ecosystem of the region. Many free-living microorganisms responsible for biomass degradation, organic matter decomposition, biogeochemical cycles, some of clinical interest, are adapted to live in the lake reservoir. This research aims to obtain bacterial genomes by metagenomic data and identify functional profiles and microbial metabolisms in the lake. Water and sediment samples were collected in the lake of the Tucuruí Hydroelectric Power Plant in the photic, aphotic and sedimentary layers of the station upstream of the new division. After filtering through a nitrocellulose membrane, the DNA samples were extracted. Total metagenome sequencing was performed on the Ion ProtonTM platform. Megahit was used to assemble the metagenomes. ORFs were predicted using Prodigal and BUSCO was used for the quantification of completeness metrics for comparative evaluation of single-copy universal orthologues. Genomes assembled into metagenomes were classified in the databases. The phyla were defined at the genus and species level, where a diverse and extremely conserved microbiota was found. The functional profile of the metagenomes found corroborated with other studies of metagenomes of oligotrophic lakes, demonstrating high biochemical, conserved and diverse activity, being a pioneering study when assembling the first metagenomes of samples from the Tucuruí Hydroelectric Power Plant.Item Acesso aberto (Open Access) Um mecanismo molecular compartilhado na padronização de nadadeiras em peixes pulmonados e membros em tetrápodes(Universidade Federal do Pará, 2019-05-03) AMARAL, Danielson Baia; SCHNEIDER, Igor; http://lattes.cnpq.br/4453558334183860The first known tetrapods to emerge already display a specifically arranged limb, composed of proximal endoskeletal elements (humerus, radius and ulna) plus distal elements (wrist bones and digits). While limbs with digits have been regarded as an evolutionary novelty, part of their skeletal organization was already in place in fish fins, as the closest relatives to tetrapods, sarcopterygian fishes, possess three proximal bones and a distal endoskeletal region composed of segmented radials. Among modern lungfish, the genus Neoceratodus has retained distal radials whereas Lepidosirenids (Protopterus e Lepidosiren) have lost them. Genetic or pharmacological inactivation of Sonic Hedgehog signaling (SHH) in developing limbs and fins leads to loss of distal endoskeleton, digits or distal radials, respectively. Shh expression during appendage development is controlled by a highly conserved enhancer element termed ZRS, present in all vertebrates including snakes. Here, we investigate the role of SHH signaling during lungfish fin regeneration by: (i) cloning and in silico analysis of ZRS from lungfish species of the three living genera; (ii) Semiquantitative PCR for Shh, Ptch1 and Gli1 and (iii) pharmacological modulation of SHH signaling during lungfish fin regeneration. Comparative analysis of vertebrate ZRS sequences revealed a 17 bp deletion of a key ETS transcription factor binding site in Lepidosirenid lungfish but not in Neoceratodus. Furthermore, as seen in salamander limb regeneration, SHH signaling is activated and is necessary for lungfish fin regeneration. Last, we show that SHH activation increases the number of distal radials in lungfish fin. Overall, our data suggests that SHH signaling, a key pathway underlying digit development in tetrapods, also controls distal radial development in lungfish fins, providing support for the homology of digits and distal radials.Item Acesso aberto (Open Access) Repercussões das análises de bioinformática nos resultados da expressão diferencial de genes e seus reguladores miRNAs no câncer gástrico(Universidade Federal do Pará, 2018-09-13) ARAÚJO, Emily Vanessa Nascimento; SANTOS, Ândrea Kely Campos Ribeiro dos; http://lattes.cnpq.br/3899534338451625The cancer, according to Globocan, in 2018, was responsible for a rate of 8.2 million deaths and about 14.1 million new cases. Regarding the analysis of gastric cancer (GC), in the state of Pará, it was estimated that for the year 2018 the number of affected men would be 23.30 and 11.45 for women. In the development of gastric adenocarcinoma, several significantly increased molecular alterations were identified when compared to non-cancerous tissues. One of these elements are miRNAS, small non-coding RNAs that act on the regulation of gene expression and can act as oncogenes or tumor suppressors. The objective of this study was to investigate the possibility of genes from tumor samples (deposited in The Cancer Genome Atlas - TCGA database) to be modified by differentially expressed miRNAs. The analysis was performed by sequencing platform, RNA-Seq, and used the software R-peridot in differential expression analysis, whose result identified six miRNAs. Next, the miRTargetLink Human platform identified eight miRNA target genes between tumor samples and their corresponding adjacent CG tissues. Functional analyzes identified important genes that can be enriched by three pathways associated with cancer: focal adhesion, RAP1 signaling pathway and calcium signaling pathway. Finally, the use of the Z-Score calculation confirmed significant findings.