Aminas bioativaas, bactéria lática e B-caroteno durante o processamento do tucupi

Abstract

The cassava roots can generate the most diverse products, but a sauce of yellow color with flavor and exotic aroma, with countless potential use in the food industry, has gained prominence in the domestic and international market. However, its production follow a popular way, since the technological information on the main stages of production, from the fermentation of the Manipueira to the final product, are still insufficient to produce a product standardized with safety and quality. This broth is the Tucupi, obtained from the fermentation of the Manipueira, is a liquid residue from the production of flour, followed by cooking. In view of the lack of data in the scientific literature about this product, a reserach of tucupis marketed in Belém (PA) was carried out and the results showed that this product is still marketed outside the pattern of identity and quality for the Tucupi. With respect to physicochemical properties, the samples showed that the processing of the product is still variable, especially in the stages of fermentation and cooking, with variations in pH, acidity and contents of total sugar and reducer. The samples showed high levels of total cyanide (8.87- 114.66 mg HCN/L) and free (0.80-38.38 mg HCN/L) and the presence of biogenic amines (tyramine, putrescine, histamine and tryptamine) which may cause food poisoning. The influence of Manipueira fermentation on the profile of carotenoids and bioactive amines were verified during the production of Tucupi. The carotenoids were not influenced by this process, and the bioactive amines identified (Spermidine, putrescine, tyramine and histamine) allowed to affirm that during the fermentative process there should be more effective control of the sanitary hygiene conditions. Based on these results, the molecular identification of the microorganisms responsible for the spontaneous fermentation of Manipueira to obtain the Tucupi. Such knowledge will enable the development of a starter culture suitable for the production of this product with quality and safety. It was highlighted for the lactic bacteria, because these microorganisms predominate during the fermentation of cassava, besides possessing genes to synthesis of biogenic amines. Were identify only two species of lactic bacteria, Lactobacillus plantarum and Lactobacillus fermentum, with predominance of L. fermentum during the whole process was identified, and the bioactive amines identified (putrescine, histamine, spermidine) did not interfere in the activity of these bacteria. The literature evidences a correlation between the identified lactic bacteria and the production of biogenic amines, but it is essential that other genetic researches performed to ratify the capacity of L. Plantarum and L. Fermentum to encode the decarboxylase enzyme in order to produce biogenic amines. Suggested that the research of active yeast in this fermentation process be carried out, because other ways on the tucupi still need to be analyzed in order to have an effective adjust in the production, to produce a safe food.