Produção in vitro de embriões bubalinos na ilha do Marajó

Abstract

Aiming to study reproductive biotechniques that can improve in vitro embryo production in the Amazon. The experiment aimed to analyze the effects of rainfall levels, semen used, cryoprotectants (diluters) of semen, presence of the dominant follicle and corpus luteum on the quantity and quality of oocytes and on the rate of embryos The experiment was carried out on the Paraíso farm in the municipality of Cachoeira do Arari, Ilha do Marajó - PA, for one year. Follicles with a diameter greater than 4.0 mm were obtained by the technique of follicular aspiration – Ovum Pick-up (OPU), guided by ultrasound. Forty buffaloes were selected for OPU and three sires for seminal collection, all of the Murrah breed and of reproductive age. For cryopreservation, the BotuBov® and Tes-Tris extenders were tested. The cumulus oophorus complexes (CCOs) were processed in the in vitro production laboratory of the Escola de Ensino Técnico do Estado do Pará (EETEPA). The best ones (CCOs) were matured, fertilized and cultured in vitro, after six days, the embryos were qualified and quantified, using the technique developed by the FIV laboratory at UFPA. The BotuBov® extender was the extender used in the PIVE for presenting better progressive motility (p<0,05) (70,70% ± 10,90%) in relation to the Tes Tris (60,42%±6,00%). There was no statistical difference (p>0,05) in the means and standard deviation of total aspirated follicles (11,75±5,23), of aspirated follicles per animal (11,23±1,39), of total screened COCs (10,5±61,65) and of CCOs tracked per animal (5,96±1,56) between the rainy and less rainy seasons (total follicles aspirated 12,47±4,86, follicles aspirated per animal 11,74 ±1,04, total screened CCOs 118,5±12,02 and screened CCOs per animal 7,27±0,83) respectively. Likewise, there was no significant difference (p>0,05) in the presence of the corpus luteum and dominant follicles and in the body condition score (ECC). In terms of embryos, there was also no statistical difference (p>0,05), between the rainiest (25,25% ±0,64%) and less rainy seasons (19,37% ±14,06%), respectively. We did not find statistical differences in embryo rates (p>0,05) in relation to the semen used (59,33% ±22,27%, 38,29% ±20,35% 54,17% ±31,55%). Thus, we concluded that the variables studied did not influence the production of embryos.