Caracterização farmacognóstica e avaliação antifúngica das folhas de Chrysobalanus icaco (Lin) em espécies de Candida

Abstract

Currently, Candidiasis has stood out among fungal infections, due to its high incidence and mortality. In parallel, there is a greater record of microbial resistance and therapeutic failures presented by antifungals available. Chrysobalanus icaco Lin, a species native to the Amazon, has been popularly used in mycoses, without scientific evidence, so the importance of research to evaluate their antifungal properties. The analysis pharmacognostic of leaves of C. icaco indicated levels of loss on drying, total ash and ash insoluble 12.3% ± 0.0288, 4.31% ± 1.67% ± 0.0001 and 0.0012, respectively. Phytochemical screening of the hydroalcoholic extract of the leaves of C.icaco (EHCI) revealed the presence of organic acids, sugars, saponins, catechins, depsídeos, phenols, flavonoids, tannins, proteins, amino acids and purine. In HPLC analysis, it was dominated by the flavonoids, especially Myricetin. Antifungal activity was tested by microdilution opposite strain ATCC 40175 Candida albicans and eleven clinical isolates of oral Candida albicans, C. dubliniensis, C. parapsilosis and C. tropicalis. The MIC ranged from greater than 6.25 mg / mL to 1.5 mg / mL and CFM, when determined, was equal to or greater than 6.25 mg / mL. The EHCI showed moderate activity against the strain ATCC 40175 and the weak antifungal activity against clinical isolates mouth. These results open new perspectives for studies that investigate fractions and substances Chrysobalanus icaco with greater activity against Candida species.