Please use this identifier to cite or link to this item: http://repositorio.ufpa.br/jspui/handle/2011/3643
Compartilhar:
Type: Artigo de Periódico
Issue Date: Jul-2005
Authors: LAURENTINO, Rogério Valois
LOPES, Ivina Giselle Lima
AZEVEDO, Vânia Nakauth
MACHADO, Luiz Fernando Almeida
MOREIRA, Márcio Ronaldo Chagas
SOUZA, Lia Lobato Batista de
ISHAK, Marluísa de Oliveira Guimarães
ISHAK, Ricardo
VALLINOTO, Antonio Carlos Rosário
Title: Molecular characterization of human T-cell lymphotropic virus coinfecting human immunodeficiency virus 1 infected patients in the Amazon region of Brazil
Citation: LAURENTINO, R. V., et al. Molecular characterization of human T-cell lymphotropic virus coinfecting human immunodeficiency virus 1 infected patients in the Amazon region of Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 100, n. 4, p. 371-376, jul. 2005. Disponível em: <http://www.scielo.br/pdf/mioc/v100n4/v100n4a06.pdf>. Acesso em: 03 abr. 2013. <http://dx.doi.org/10.1590/S0074-02762005000400006>.
Abstract: The present work evaluated the epidemiology of human immunodeficiency virus 1/human T-cell lymphotropic virus (HIV-1/HTLV) coinfection in patients living in Belém (state of Pará) and Macapá (state of Amapá), two cities located in the Amazon region of Brazil. A total of 169 blood samples were collected. The sera were tested by enzyme-linked immunosorbent assay to determine the presence of antibodies anti-HTLV-1/2. Confirmation of infection and discrimination of HTLV types and subtypes was performed using a nested polymerase chain reaction targeting the pX and 5' LTR regions, followed by restriction fragment length polymorphism and sequencing analysis. The presence of anti-HTLV1/2 was detected in six patients from Belém. The amplification of the pX region followed by RFLP analysis, demonstrated the presence of HTLV-1 and HTLV-2 infections among two and four patients, respectively. Sequencing HTLV-1 5' LTR indicated that the virus is a member of the Cosmopolitan Group, Transcontinental subgroup. HTLV-2 strains isolated revealed a molecular profile of subtype HTLV-2c. These results are a reflex of the epidemiological features of HIV-1/HTLV-1/2 coinfection in the North region of Brazil, which is distinct from other Brazilian regions, as reported by previous studies.
Keywords: HIV-1
Vírus linfotrópico de células T humanas tipo 1
Diagnóstico laboratorial
Epidemiologia molecular
Vírus 2 linfotrópico T humano
Belém - PA
Macapá - AP
Pará - Estado
Amapá - Estado
Amazônia brasileira
ISSN: 1678-8060
0074-0276
metadata.dc.rights: Acesso Aberto
Appears in Collections:Artigos Científicos - ICB

Files in This Item:
File Description SizeFormat 
Artigo_MolecularCharacterizationHuman.pdf102.69 kBAdobe PDFView/Open


This item is licensed under a Creative Commons License Creative Commons