Please use this identifier to cite or link to this item: http://repositorio.ufpa.br/jspui/handle/2011/6875
metadata.dc.type: Artigo de Periódico
Issue Date: 2009
metadata.dc.creator: CHRISTOFOLETTI, Cintya Aparecida
DAVID, José Augusto de Oliveira
FONTANETTI, Carmem Silvia
Title: Application of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparison
Citation: CHRISTOFOLETTI, Cintya Aparecida; DAVID, José Augusto Oliveira; FONTANETTI, Carmem Silvia. Application of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparison. Genetics and Molecular Biology, São Paulo, v. 32, n. 1, p. 155-158, 2009. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100024&lng=pt&nrm=iso>. Acesso em: 8 set. 2015. <http://dx.doi.org/10.1590/S1415-47572009005000020>.
Abstract: The present study applied the comet assay to erythrocytes of Oreochromis niloticus with the aim of improving protocols to detect DNA damage in these cells, by using two distinct pHs (pH = 12.1 and pH > 13) and evaluating whether there is a correspondence between silver and ethidium bromide staining. Comets were visually examined and, the frequency of cells with and without damage was obtained, as well as the distribution of classes and scores. By using the Kruskal-Wallis test, our results revealed that pH 12.1 is more effective, although both pHs can be used. Our findings also suggest that silver staining can substitute ethidium bromide, an expensive and highly toxic stain that requires specific equipment for examination.
Keywords: Peixe
Tilápia-do-Nilo
Oreochromis niloticus
DNA
Ensaio cometa
Impregnação pela prata
Etídio
ISSN: 1678-4685
metadata.dc.rights: Acesso Aberto
Appears in Collections:Artigos Científicos - ICB

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