Dissertações em Ciência e Tecnologia de Alimentos (Mestrado) - PPGCTA/ITEC

URI Permanente para esta coleçãohttps://repositorio.ufpa.br/handle/2011/8949

O Mestrado Acadêmico em Ciência e Tecnologia de Alimentos teve início em 2004 e funciona no Programa de Pós-Graduação em Ciência e Tecnologia de Alimentos (PPGCTA) do Instituto de Tecnologia (ITEC) da Universidade Federal do Pará (UFPA).

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Agora exibindo 1 - 9 de 9

Acesso aberto (Open Access)
Adição de ácido esteárico e surfactante em filmes elaborados com proteínas miofibrilares obtidas de corvina (micropogonias furnieri).
(Universidade Federal do Pará, 2017-03-03) VIEIRA, Lorena Limão; LOURENÇO, Lúcia de Fátima Henriques; http://lattes.cnpq.br/7365554949786769
The interest in biodegradable films as food packaging is a worldwide trend that has increased significantly in recent years. The myofibrillar proteins, particularly fish ones, have been gaining interest in film technology. The films made from polysaccharides or proteins have excellent mechanical and optical properties, but these films have a high water vapor permeability (WVP). The incorporation of hydrophobic substances, such as stearic acid, into the film-forming solution, is an alternative to increase the barrier properties of the film to water vapor. But to facilitate the incorporation of the lipid in the protein matrix, it is necessary to add surfactant, which are substances capable of interacting with the protein and the fatty acid. The objective of this work was to develop and characterize films of myofibrillar proteins obtained from corvina waste (Micropogonias furnieri) using glycerol as plasticizer, sodium dodecyl sulfate (SDS) as surfactant and stearic acid (SA) as lipid, to improve the technological properties of the film. The characterization of the waste and lyophilized myofibrillar proteins (MP) was performed, indicating that the raw material had a high protein content (96.03% b.s.), important for the formation of the biopolymer matrix. A complete factorial planning was done to define the region of better mechanical, physical and barrier properties of films. The optimized biofilm was prepared with 2.84% MP, 3.18% SA, and 78.41% SDS and 30% glycerol. Concentrations of SA and SDS significantly reduced the WVP, reaching 5.87E-11 g m m-² s-¹ Pa-¹, representing a reduction of 31% when compared to the control biofilm. The mechanical properties of the optimized biofilm were favored, presenting 235.60% of elongation and 6.35 MPa of tensile strength producing stronger and more flexible films. The values of transparency were high, indicating tendency to opaque and yellowish tone, however, with excellent UV barrier properties and it can be used in light sensitive foods. The optimized film also presented good thermal stability and the microstructure revealed a structural change in the filmogenic matrix, in relation to the control, with presence of grooves and protuberances on the surface, confirmed by X-ray diffraction, which indicated the influence of SDS and SA on crystallinity of the film. A 22% increase in solubility and a slight decrease in the swelling of the optimized film were observed.
Acesso aberto (Open Access)
Avaliação dos efeitos da desodorização da gelatina de pele de pescada amarela (Cynoscion ocupa) por diferentes métodos
(Universidade Federal do Pará, 2023-04-14) RABELLO, Fernanda Sales; LOURENÇO, Lúcia de Fátima Henriques; http://lattes.cnpq.br/7365554949786769
Fish skins are considered good sources to extract high quality gelatine for applications in the food industry, however, this fish gelatine may have a characteristic odor and taste, causing limitations for its application, especially as a food ingredient and in supplements. Several methodologies have been studied in order to remove or reduce the odor in products made with fish. Among these techniques, we can find degreasing and adsorption. The objective of this research was to deodorize the gelatine from the skin of yellow hake (Cynoscion acoupa) using three different methodologies: activated charcoal, ethanolic delipidation and their combination. Gelatin was obtained from the immersion of the skins in acidic and alkaline solutions. The desorization by delipidification occurred with the use of an ethanol solution (1:2) and the adsorption made use of activated charcoal in powder (0.5%) and in grain (0.7%). Analyzes were carried out to evaluate the physical-chemical, technological and sensorial quality of the gelatins. The analysis results were submitted to ANOVA and Tukey Test (p <0.05). All physicochemical parameters analyzed in the present study showed a significant difference between the studied variables, except for proteins, lipids and moisture. The variables of emulsifying capacity, gel strength, melting point showed a significant difference between the deodorization methodologies in relation to the control treatment. The EGAC treatment influenced the higher luminosity of the samples, presenting negative values in the a* parameter. The treatment with powdered activated charcoal influenced the lower chromaticity and lower yellowish tone. According to the electropheretic profile, samples E, ACP and EGAC showed collagen α and γ bands. From the FTIR analysis, the presence of activated carbon in the desorizations (ACP, GAC and EGAC) resulted in lower interactions with the amine group. In the sensorial evaluation, the samples deodorized with activated charcoal behaved similarly in the sensorial evaluation, highlighting the behavior of the EGAC sample.
Acesso aberto (Open Access)
Efeito da adição de cera de abelha sobre as propriedades de filmes biodegradáveis elaborados com gelatina da pele de peixe
(Universidade Federal do Pará, 2022-08-22) MORAES, Vinicius Sidonio Vale; TEIXEIRA, Camilo Barroso; http://lattes.cnpq.br/7059706802675228; LOURENÇO, Lúcia de Fátima Henriques; http://lattes.cnpq.br/7365554949786769; https://orcid.org/0000-0001-5009-8235
Biodegradable films are defined as thin materials made from biomolecules, which act as a barrier against moisture, gases, light and mechanical injuries and, consequently, extend the shelf life of products. Biodegradable films made from biomolecules have gained visibility today, especially those made from gelatin extracted from fish skin. Despite having good optical and mechanical properties, they have high water vapor permeability (PVA). The addition of lipids such as beeswax is a good option to solve this problem. The objective of this work was the elaboration and characterization of a biodegradable film from yellow hake skin gelatin added with beeswax using sodium dodecyl sulfate (SDS) and stearic acid as a surfactant, aiming to improve the technological properties of the film. A complete factorial design was carried out to define the region with the best barrier and solubility of the films. The planning indicated the tendency of the concentrations for the best results of the characteristics of the film. Based on this, four films of film-forming solution containing 130 ml were prepared, the first being a Control film composed of 2% gelatin and 20% glycerol; second film (F1) composed of 2% gelatin, 20% glycerol, 5% beeswax, 80% SDS and 100% stearic acid; third film (F2) composed of 2% gelatin, 20% glycerol, 10% beeswax, 80% SDS and 100% stearic acid and the fourth film (F3) composed of 2% gelatin, 20% glycerol, 15% beeswax, 80% SDS and 100% stearic acid. The thickness of the films presented heterogeneous results due to the poor incorporation of the wax in the film matrix. Likewise, the addition of beeswax to the film did not have a positive influence on PVA because of the appearance of bubbles in the film-forming solution. However, all films obtained excellent solubility results, justified by the high hydrophobicity of the wax. The films obtained results that indicate opaque films with greenish yellow tones and high luminosity, with an excellent UV barrier, and can be used in foods with sensitivity to light. The microstructure analysis showed inhomogeneous films, with bi-layer-like structures, free spaces and cracks in the matrix.
Acesso aberto (Open Access)
Estudo da influência do extrato purificado do açaí (euterpe oleracea) em filmes de gelatina e seu comportamento como indicador de pH
(Universidade Federal do Pará, 2019-05-14) BRITO, Raíra Sá de; LOURENÇO, Lúcia de Fátima Henriques; http://lattes.cnpq.br/7365554949786769; https://orcid.org/0000-0001-5009-8235
Acesso aberto (Open Access)
Estudo do processo de modificação química do amido na farinha de banana verde da variedade nanicão (Musa sp. AAA)
(Universidade Federal do Pará, 2014-06-27) NOGUEIRA, Francylla Milhomens; LOPES, Alessandra Santos; http://lattes.cnpq.br/8156697119235191; LOURENÇO, Lúcia de Fátima Henriques; http://lattes.cnpq.br/7365554949786769
Acesso aberto (Open Access)
Estudo dos riscos e benefícios associados ao consumo de três espécies de peixes da Amazônia
(Universidade Federal do Pará, 2019-09-20) GOMES, Luciana Cristina Mancio; LOURENÇO, Lúcia de Fátima Henriques; http://lattes.cnpq.br/7365554949786769
Fishing is one of the most explored activities in the amazon region, fish is an important protein source, however, it can represent one of the main vehicles of contamination due to its great capacity to bioaccumulate substances in tissues such as muscle and organs, being considered excellent bioindicators of the species. Its habitat. The aim of the present work was to study the amazonian species, curimatã (prochilodus nigricans), goby hake (macrodn ancylodon), and pyramutaba (brachyplatystoma vaillantii) by investigating the risks or benefits associated with consumption. In the muscle tissue and liver of the fish in different seasonal periods (rainy and dry season) amino acid and fatty acid profile analyzes were performed by liquid and gas chromatography. The determination of essential elements (cu, fe, mg, na and zn) was quantified by flame atomic emission spectrometry and the contaminants, lead (Pb) and mercury (Hg) by graphite furnace atomic absorption spectrometry. The validation of the methodology was performed through the addition and recovery method. The results obtained in the muscle and liver were correlated with biometric data, species feeding habits and the limits established by the national health surveillance agency for human consumption. The calculation of the tolerable weekly intake index (PTWI) was applied for the toxic elements pb and hg in different seasonal periods. According to the results, the species presented oleic (n-9), linoleic (omega-6) and arachidonic (omega-6) polyunsaturated fatty acids in the muscle, whereas in the liver the three species were c18:0, c18:1ω9, c18:2ω6, c20:4ω6, c22:6ω3. The total saturated fatty acid contents identified were higher in piramutaba, polyunsaturated in hake and monounsaturated in curimatã. Regarding the amino acid profile of the fillets, the highest predominance was histidine, alanine and serine. The species studied presented high levels of monounsaturated and polyunsaturated fatty acids. The monounsaturated ones presented higher contents in the curimatã species and the polyunsaturated ones were higher in the hake, both in the muscle and liver. Regarding minerals, calcium levels were quite significant between species in different seasonal periods, being predominant in the dry season, while Fe, Cu, Na and Zn presented higher levels in the rainy season. In the liver, during the rainy season, Mg, Zn and Cu values had average concentrations in curimatã specimens. While in the dry season the ca, mg, na and zn contents had higher predominance among the species. Regarding contaminants, pb levels were higher than the limit allowed by the legislation, showing significant behavior in relation to seasonality. The hg content in the three species was higher in the rainy season, the curimatã species reached higher levels in the muscle. In the liver, the levels of Hg in the rainy season were above the limit recommended by the legislation. In the dry season, the curimatã species contributed the most with 131.79%, exceeding the tolerable weekly limit of hg consumption, being considered inappropriate. According to the estimated weekly intake of toxic elements (PTWI) during the rainy season, acceptable limits were found among the contaminants. However, in the dry season, curimatã contributed the most with 131.79%, exceeding the tolerable weekly consumption limit. From Hg. It is concluded that the constant biomonitoring of the species commercialized in Belém-PA and surroundings that may present some contamination by toxic elements is necessary, thus avoiding possible reflexes and the compromise of human health and fish reproductive process.
Acesso aberto (Open Access)
Extrato polissacarídico de cogumelo Pleurotus ostreatus pode melhorar as propriedades tecnológicas do filme de gelatina de peixe
(Universidade Federal do Pará, 2020-02-19) FREITAS, Maurício Madson dos Santos; LOURENÇO, Lúcia de Fátima Henriques; http://lattes.cnpq.br/7365554949786769
The aim of this papert was to evaluate the influence of the polysaccharide extract (POP) obtained from the mushroom Pleurotus ostreatus on the technological properties of fish gelatin films. The optimization conditions of this study were: 3.0% gelatin (GA), 6.26% plasticizer and 1.0% polysaccharide extract (POP). Under these conditions it was possible to obtain values of 5.30x10-11 g.m.m-2, s-1.Pa-1 of WPV, 17.78% of tensile strength and 15.56% of elongation. The optimal film (POP-GA) showed antioxidant activity, adequate visual and structural characteristics, as demonstrated by FTIR, XDR and SEM analyzes. Therefore, the technological properties found in the film with polysaccharide extract and gelatin, have potential for application in the food industry, as well as in the pharmaceutical industry.
Acesso aberto (Open Access)
Propriedades tecnológicas e antimicrobiana de biofilmes de proteínas de peixe com óleo essencial de cravo.
(Universidade Federal do Pará, 2017-09-18) SILVA, Wagner Barreto da; LIMA, Consuelo Lúcia Sousa de; http://lattes.cnpq.br/2971385954203459; LOURENÇO, Lúcia de Fátima Henriques; http://lattes.cnpq.br/7365554949786769
The objective of this work was to evaluate the antimicrobial capacity of biofilms elaborated with proteins extracted from filleting residues of gilt (Brachyplatysoma roussauxii) added with essential oil of clove (Eugenia caryophyllata), besides analyzing the technological properties of the films. Two biofilms were prepared, one with 2% of myofibrillar proteins extracted from fillets and the other with 2% of gelatin of the skin of gilt, by the casting method. The concentrations of clove essential oil were 0% (white), 1%, 1.5% and 2% relative to the volume of solution. In the films, the antimicrobial activity was evaluated by the disc-diffusion method and mechanical analyzes (elongation and tensile strength), barrier (permeability to water vapor, water solubility and swelling index), thickness, color and characteristics morphological and structural analyzes using scanning electron microscopy (SEM). Myofibrillar proteins and gelatins extracted from gilt shavings and skin are excellent raw materials for application in biofilms, with protein content of 94.92% and 79.67% and yield of 14.10% and 32.92%, respectively. The biofilms added clove essential oil presented excellent values of water vapor permeability and high elongation values contributing to a better application in food, as well as yellow coloration with low intensity. The biofilms of myofibrillar proteins had an inhibitory effect on the concentrations used against Gram positive bacteria, Staphylococus aureus and Enterococus fecalis. Gelatin biofilms added with OEC did not inhibit the growth of Gram-positive and Gram-negative bacteria.
Acesso aberto (Open Access)
Revestimento e filme biodegradável de gelatina de peixe e óleo de açaí: efeito da aplicação na qualidade pós colheita de tomate
(Universidade Federal do Pará, 2021-01-13) SILVA, Ana Caroline Pereira da; LOURENÇO, Lúcia de Fátima Henriques; http://lattes.cnpq.br/7365554949786769
Tomato (Solanum lycopersicum) is a climacteric fruit that has a short post-harvest life due to biochemical reactions that accelerate maturation and senescence, affecting its quality. Biodegradable films have been studied as an alternative to minimize posthar-vest losses of fruits and prolong the shelf life of these foods. These coatings, when made with fish gelatin, have high water vapor permeability (PVA) and solubility due to their hydrophilic nature. And the addition of vegetable oils can improve these technological properties in addition to making the packaging active, acting as antioxidants and antimi- crobials. The aim of this study was to evaluate the influence of the addition of açaí oil on the technological properties of gelatin films and coatings and to apply the coating on tomatoes to preserve their postharvest quality. Gelatin extracted from fish skins had a yield of 18%, gel strength of 244g and melting point of 28 °C, important technological properties for this biopolymer to be used in the preparation of packaging. Two factorial planning were carried out to define the optimized conditions for obtaining the coating and the superimposition of graphics to define the conditions for the elaboration of the composite film. The coating with 5.40% gelatin, 17.25% açaí oil and 18.00% plasticizer showed the best responses for high viscosity and higher gel strength. The film with 5.40% gelatine, 21.50% açaí oil and 18.00% plasticizer showed the best responses for low water vapor permeability and high tensile strength. The PVA values obtained were 16.83x10-8 and 12.73x10-11 g.m-1. s-1. Pa-1 for control film (without oil) and composite (with oil), respectively. The composite film showed excellent results for the technological properties that are important for application to fruit. The optimized coating was efficient to maintain the quality characteristics of the tomatoes, as it reduced the percentage of mass loss and obtained a higher percentage of acidity compared to the control, proving that the coating with açaí oil was effective in delaying the ripening processes. It is concluded that the coating of fish gelatin and açaí oil extended the shelf life of tomatoes, being a promising alternative as a packaging for climacteric fruits.

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