Programa de Pós-Graduação em Ciências Farmacêuticas - PPGCF/ICS

URI Permanente desta comunidadehttps://repositorio.ufpa.br/handle/2011/2312

O Programa de Pós-Graduação em Ciências Farmacêuticas (PPGCF) vinculado ao Instituto de Ciências da Saúde (ICS) da Universidade Federal do Pará (UFPA) apresenta um auto-impacto de inserção regional uma vez que se trata do único PPGCF na Região Norte pelo grande potencial de utilização da biodiversidade na região amazônica. Além de favorecer a fixação e atração de profissionais qualificados na área de Ciências Farmacêuticas na Região Amazônica.

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    Exposição ao MEHG provoca dano na medula espinhal: percepções a partir da análise proteômica e estresse oxidativo
    (Universidade Federal do Pará, 2020-08-27) EIRÓ, Luciana Guimarães; LIMA, Rafael Rodrigues; http://lattes.cnpq.br/3512648574555468; https://orcid.org/ 0000-0003-1486-4013
    Methylmercury (MeHg) is considered by the World Health Organization as one of the chemicals of greatest public health concern. Thus, knowing the susceptibility of central nervous system regions and the absence of evidence about the effects on the spinal cord, this study aimed to investigate proteomic and biochemical changes in the spinal cord after MeHg long-term exposure at low doses. For this, male Wistar rats were exposed to a dose of 0.04 mg/kg/day by for 60 days. After that, the proteome was identified with subsequent overrepresentation analysis (ORA). For the oxidative biochemistry, the antioxidant (ACAP, TEAC, GSH) and pro-oxidants (LPO and nitrite ions) parameters were evaluated. The proteomic analysis showed several altered proteins that participate in biological processes, cellular components, and molecular functions. There was an increase in total mercury (Hg) levels in the spinal cord, as well as an increase in LPO and nitrite ions and a reduction in ACAP, TEAC and GSH. Therefore, exposure to low doses of MeHg can trigger oxidative stress associated with changes in the proteomic profile.
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    A exposição prolongada ao fluoreto durante a adolescência à fase adulta modula o proteoma hipocampal e gera danos cognitivos associados a um padrão neurodegenerativo no hipocampo de camundongos
    (Universidade Federal do Pará, 2021-11-19) BITTENCOURT, Leonardo de Oliveira; GOMES, Bruna Puty Silva; http://lattes.cnpq.br/3013433540155834; https://orcid.org/ 0000-0001-5570-8530; LIMA, Rafael Rodrigues; http://lattes.cnpq.br/3512648574555468; https://orcid.org/ 0000-0003-1486-4013
    Fluoride (F) is used in artificial water fluoridation due to its anticariogenic activity, but it is also present in soils and natural reservoirs due natural high levels or anthropogenic actions, featuring it as a potential environment toxicant. In addition to skeletal and dental fluorosis in people living in regions with high F levels, some studies have explored the association between F exposure and cognitive damages, and although the evidences have indicated that only high levels pose a deleterious effect on cognition, a heated debated has grown regarding the safety of water supply artificial fluoridation. In this way, this study investigated whether long-term F exposure, from adolescence to adulthood, under the paradigms of water fluoridation and environmental issue, would be associated with memory and learning impairments in mice, and unravel molecular and morphological aspects involved. For this, 21-days-old mice received for 60 days, 10mg/L or 50mg/L of F in drinking water; the control group received only water without addition of F. Then, the cognitive functions were assessed by the Morris Water Maze and Inhibitory Step-down Avoidance test, followed blood and hippocampus collection for plasma F level determination, and hippocampal global proteomic profile analysis by Mass Spectrometry. Some animals were perfused for immunohistochemical analyses of mature neurons density in CA1, CA3, hilus and dentate gyrus (DG) regions. The results indicated that prolonged exposure to F from adolescence to adulthood increased plasma F bioavailability, and the higher F concentration triggered short- and long-term memory impairments, being associated with the modulation of the global proteomic profile, and a neurodegenerative pattern in the CA3 and DG regions. Our data, in a translational perspective, gives evidences of potential molecular targets of F neurotoxicity in the hippocampus and reinforces the safety of low fluoride concentration exposure, besides the need for attention of people living in endemic regions.
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