Dissertações em Biotecnologia (Mestrado) - PPGBIOTEC/ICB
URI Permanente para esta coleçãohttps://repositorio.ufpa.br/handle/2011/6090
O Mestrado em Biotecnologia teve início em 2011 e funciona no Programa de Pós-Graduação em Biotecnologia (PPGBIOTEC) do Instituto de Ciências Biológicas (ICB) da Universidade Federal do Pará (UFPA).
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Item Acesso aberto (Open Access) Influência dos metabólitos secundários de Piper divaricatum da região amazônica no controle do Fusarium solani f. sp. piperis causador da fusariose em pimenta do reino(Universidade Federal do Pará, 2014-09-18) MEIRELES, Erisléia das Neves; RAMOS, Alessandra de Rezende; http://lattes.cnpq.br/1279694874191138; SILVA, Joyce Kelly do Rosário da; http://lattes.cnpq.br/2278686174214080The culture of black pepper (Piper nigrum L.) is one of the main agricultural activities in Pará State and it suffers serious damages caused by fusariosis, disease restrict in Brazil. This paper evaluated the antifungal activity in vitro of essential oils of species of Piper rich in phenylpropanoid before the Fusarium solani f. sp. piperis, which causes the fusariosis. The inhibition of mycelial growth by the method of agar dissemination in concentration of 5 mg.mL-1 was considered: decrease to P. aduncum (20.3%) and P. Krukoffii (31.4%); moderated to P. callosum (55.7%) and P. marginatum (70.3%) and high to P. divaricatum (93.3%). The major components identified by CG-EM were dilapiol (92.0%), safrole (78.0%), methyleugenol (75.2%) and eugenol (7.9%), apiol (80.0%), Z-isoosmorizol (44.0%) and E-anethole (22.0%), respectively. The oil of P. divaricatum and its major compounds present CIM values of 0.75 mg.mL-1. The evaluation of the effects combined of eugenol and methyl eugenol pointed eugenol as the main responsible by the activity.Item Acesso aberto (Open Access) Produção e avaliação da atividade antioxidante de metabólitos secundários de Piper divaricatum G. Meyer sob diferentes condições de cultivo(Universidade Federal do Pará, 2015-08-06) CORPES, Rosana Silva; SILVA, Joyce Kelly do Rosário da; http://lattes.cnpq.br/2278686174214080Many species of the genus Piper are widely distributed in the Amazon and various biological applications because of large structural diversity of its secondary metabolites. The species Piper divaricatum, is endemic in the Amazon and produces in its essential oil high concentrations of methyleugenol (50-90%), an phenylpropanoid with antioxidant and fungicidal properties. Because of its potential applications, the objective of this study was to establish the in vitro cultivation and comparing the biosynthesis of secondary metabolites and antioxidant properties with the in vivo cultivation. For establish the in vitro culture were used shoot apexes on Murashige e Skoog medium with addition of regulator BAP 0.5 mg.mL-1. For in vivo cultivation, micropiles were propagated in the greenhouse in vermiculite and adding nutritious Murashige e Skoog solution. The volatile compounds identified in the leaves of seedlings grown in vivo were methyleugenol, β-elemene and E-β-ocimene, which did not differ from in vivo cultivation, with the exception of 90 days. The in vitro culture of roots was not efficient to produce phenylpropanoids and presented a very different profile compared to the in vivo cultivation of terpenes. In general, for plants in vitro cultivated there was no statistically significant difference in the phenolics compounds content and antioxidant activity in the leaves. However, the antioxidant activity of roots was significant. The results support the hypothesis that in vitro regenerated plants can synthesize metabolites similar the matrix plant and maintain their biological properties.