Dissertações em Ciências Farmacêuticas (Mestrado) - PPGCF/ICS

URI Permanente para esta coleçãohttps://repositorio.ufpa.br/handle/2011/2313

O Mestrado Acadêmico em Ciências Farmacêuticas teve início em 2005 e homologado pelo CNE e funciona no Programa de Pós-Graduação em Ciências Farmacêuticas (PPGCF) do Instituto de Ciências da Saúde (ICS) da Universidade Federal do Pará (UFPA).

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Navegando Dissertações em Ciências Farmacêuticas (Mestrado) - PPGCF/ICS por Autor "BRAZÃO, Maria Angélica Bolini"

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    Atividade antimicrobiana do óleo essencial de Piper aduncum L. e seu componente, dilapiol, frente a Staphylococcus spp. multirresistentes
    (Universidade Federal do Pará, 2012-08-31) BRAZÃO, Maria Angélica Bolini; MONTEIRO, Marta Chagas; http://lattes.cnpq.br/6710783324317390
    The essential oil of Piper aduncum (EPO) and its main component, the dilapiol (76.5%), were evaluated for antibacterial activity against different strains of Staphylococcus spp. ATCC and multiresistant. For testing the antibacterial activity of EPO and dilapiol were determined Minimum Inhibitory Concentration (MIC) and minimum bactericidal concentration (MBC), the technique of microdilution and by counting Colony Forming Units (CFU) using concentrations of 250, 500 , 750 and 1000 μg/ml of EPO and concentrations of 100 and 1000 μg/ml dilapiol. The bacterial inoculum used was adjusted to 1x104 range from 0.5 Mc Farland. As a negative control inoculum was used along with Tween 20, solubilising the essential oil and dilapiol, and as positive control we used the chloramphenicol 0.05 mg/mL. These compounds were tested against strains of S. aureus ATCC 25923, S. epidermidis ATCC 12228, MRSA hospital, and S.epidermidis, S. lentus multiresistant nosocomial. The results showed that the EPO showed the MIC 90% of 500 μg/mL and MBC of 1000 μg/mL against S. aureus ATCC 25923, whose concentration of 500 μg/mL was able to inhibit 60% of bacterial growth. The strain MRSA, the EPO showed a small inhibition (10%) at a concentration of 750 μg/mL, being obtained in the MIC90% 1000 μg/mL. In S. epidermidis ATCC 12228, the OEPA showed antimicrobial activity with MIC90% at 500 μg/mL and MBC at 750 μg/mL. For the strain S. epidermidis multiresistant, the PEO was able to inhibit only 35% growth of this strain at a concentration of 750 μg/mL, but the value obtained in the MIC90% 1000 mg/mL. As for dilapiol, the compound showed antimicrobial activity against a strain of S. aureus ATCC 25923 at a concentration of 1000 μg/mL, 100% growth inhibiting (CBM). On the other hand, had no antimicrobial effect on MRSA strain nor S. lentus multiresistant. Furthermore, the dilapiol inhibited only 20% of the growth of S. epidermidis ATCC 12228 and S. epidermidis multiresistant concentration of 1000 μg/mL. Thus, the data show a moderate antibacterial activity of the essential oil, and the dilapiol showed weak in vitro antimicrobial activity.
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