Avaliação antitumoral de amidas graxas derivadas de triglicerídeos de óleo de andiroba (Carapa guianensis aublet) em linhagem celular de glioma in vitro

Abstract

Glioma is a rare type of tumour, which acts on Nervous System in a very aggressive way, presents problems in its diagnosis, low effective treatments and survival time less than one year after diagnosis. Due to factors such as intratumoral cell variability, inefficient chemotherapy drugs, adaptive resistance development to the drugs and tumour recurrence after resection, the development of new drugs becomes necessary. In this sense, molecules analogues to endocannabinoids such as fatty amides are a good alternative, since scientific literature shows that they can act as antitumor agents through the interaction with the endocannabinoid system, which modulates many metabolic pathways related to cancer. In this work, two fatty amides synthetized from andiroba (Carapa guianensis aublet) using lipase from Candida antarctica-B (CAL-B) oil were tested aiming to evaluated its potential in the glioma treatment in vitro (C6). AGs reduced C6 cell viability in a dose dependent manner while were not toxic to normal glia cells. Both FAA1 and FAA2 caused apoptosis cell death and also loss of mitochondrial integrity probably by inhibiting PI3k/AKT pathway. Furthermore, FAAs were capable of reduce the C6 migratory potential. In conclusion FAAs have a promising potential to treat glioma-type brain cancer.