Obtenção de genomas bacterianos a partir de dados metagenômicos da usina hidrelétrica de Tucuruí

Abstract

Tucuruí is the second largest Brazilian Hydroelectric Power Plant, the artificial lake in the municipality of Tucuruí has altered the ecosystem of the region. Many free-living microorganisms responsible for biomass degradation, organic matter decomposition, biogeochemical cycles, some of clinical interest, are adapted to live in the lake reservoir. This research aims to obtain bacterial genomes by metagenomic data and identify functional profiles and microbial metabolisms in the lake. Water and sediment samples were collected in the lake of the Tucuruí Hydroelectric Power Plant in the photic, aphotic and sedimentary layers of the station upstream of the new division. After filtering through a nitrocellulose membrane, the DNA samples were extracted. Total metagenome sequencing was performed on the Ion ProtonTM platform. Megahit was used to assemble the metagenomes. ORFs were predicted using Prodigal and BUSCO was used for the quantification of completeness metrics for comparative evaluation of single-copy universal orthologues. Genomes assembled into metagenomes were classified in the databases. The phyla were defined at the genus and species level, where a diverse and extremely conserved microbiota was found. The functional profile of the metagenomes found corroborated with other studies of metagenomes of oligotrophic lakes, demonstrating high biochemical, conserved and diverse activity, being a pioneering study when assembling the first metagenomes of samples from the Tucuruí Hydroelectric Power Plant.