Obtenção de Extratos Polissacarídicos do Cogumelo Comestível Pleurotus Ostreatus com tecnologia de fluido supercrítico

Abstract

The present dissertation proposal sought to develop an efficient method for recovery of extracts rich in polysaccharides from the edible mushroom Pleurotus ostreatus using binary system with hot water and supercritical CO2. The dissertation was structured in two interconnected chapters. Chapter I is a published article of review bibliographic, in which the latest findings of the literature on the subject are addressed clearly and factually, emphasizing the development of new extraction techniques, biological potential, and new technologies. Chapter II is a research article, where the main findings about the extraction and characterization process of a fraction rich in polysaccharides are displayed. The results show that the technology and process developed were efficient for recovery with a high yield of extracts rich in polysaccharides. In addition, research has shown that the extracts are a mixture of heteropolysaccharides, β-glucans, α-glucans, and oligosaccharides. The extract with the best extraction condition has high antioxidant activity, does not have cytotoxic effects, and has cytoprotective activity against free radicals. Thus, the method developed to recover extracts rich in polysaccharides is efficient, environmentally friendly and has potential for industrial implementation.